Multi-Photon Imaging (MPI) mostly involves two-photon microscopy. MPI is a fluorescence technique that allows imaging living tissue up to a depth of one millimeter. Two-photon excitation may be a viable alternative to confocal microscopy due to its deeper tissue penetration and reduced photooxicity.
Two-photon excitation is based on the idea that two photons of low energy can excite a fluorophore in a quantum event, resulting in the emission of a fluorescence photon, typically at a higher energy than either of the two excitatory photons. The probability of the near-simultaneous absorption of two photons is extremely low. Therefore a high flux of excitation photons is required. In two-photon excitation microscopy an infrared laser beam is focused through an objective lens.. Since femtosecond fiber lasers are maintenance free, reliable, compact and low cost features, they’re becoming a very promising source for two-photon microscopy.
MPI usually needs the shortest pulse of around 100 fsec with repetition rates in the 10s of MHz and average powers in the 100s of mW, specifications covered by the Carmel-X product line.
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